A STUDY ON USING MICROBIAL SCREENING METHOD FOR DETECTION OF ANTIBIOTIC RESIDUES IN FOOD

Abstract

The abuse of antibiotics in breeding and in food preservation can lead to the existence of residual antibiotics in many products such as egg, milk, meat, fish and in the viscera that not only affects negatively the quality of the product but also threatens human health. In this research, four microorganism strains: Micrococcus Luteus ATCC 9341, Bacillus cereus ATCC 11778, Bacillus subtilis L2 and Bacillus stearоthermophilus were inoculated on 9 different culture media in order to assess the effect of common-abused antibiotics for food (tetracycline, benzylpenicillin, streptomycin, grisin, bacitracin) on the growth ability of such bacteria. The result showed that all objective bacteria were inhibited or killed in the antibiotic-containing media. Among them, Bacillis subtilis L2 cultured in the media with pH 8,0 and Bacillus stearоthermophilus cultured in the media Kundrat were the two strains which are the most sensitive to the studied  antibiotics.